Coding

Part:BBa_K3419000:Design

Designed by: Sophia Windemuth   Group: iGEM20_Cornell   (2020-10-17)


Aspartate-semialdehyde dehydrogenase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers must be designed with homologous sequences to allow for the Asd knockout, as given by the OpenWetWare protocol (Reference [2]).


Source

The sequence comes from an Escherichia coli K12 strain, from the uniprot database (UniProtKB - P0A9Q9 (DHAS_ECOLI))

References